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In this study, we used the AAA anti-arachnid antivenom (Study drug: AAA2013-BB-IND 15757; Batch number 3IT08001; protein concentration of 18.83 ± 0.03 (n = 3)). As per manufacturer’s instructions, the antivenom was made up in 2 mL deionized water and inverted until dissolved completely. Antivenom was centrifuged (10 min, 4 °C, 14,000 RCF), and the supernatant collected stored at 4 °C. Antivenom working stocks (2.6% and 7.5% antivenom) were made by diluting antivenom with an Owren Koller (OK) Buffer (Stago catalog #00360) and then stored at 4 °C.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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