Antibodies and immunoblot analyses

RT Ritesh Tandon
DM Dipanwita Mitra
PS Poonam Sharma
MM Martin G. McCandless
SS Stephen J. Stray
JB John T. Bates
GM Gailen D. Marshall
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Monoclonal anti-Ace2 antibody (AF933; R&D Systems, Minneapolis, MN) was used to detect the Ace2 protein in cell lysates. Peroxidase-labeled anti-mouse IgG (Vector Laboratories, Burlingame, CA) was used as the secondary antibody. Blots were detected using ECL Western blotting detection reagents (GE Healthcare, Buckinghamshire, United Kingdom).

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