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To get HUWE1 defective Hela cells, we transfected shRNA lentiviral constructs within LV2 (U6/Puro) (GenePharma, Suzhou, Chain) and transfected cells were then selected in puromycin (2 μg/ml) for 2 weeks. The shRNA sequences were as follows: shNC: 5′-TTCTCCGAACGTGTCACGT-3′; shHUWE1-1: 5′-CATTGGAAAGTGCGAGTTA-3′; shHUWE1-2: 5′-CTGTGAGAGTGATCGGGAA-3′.
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