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Formation of mCherry-hnRNPA2-LCD hydrogels
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Purified mCherry-hnRNPA2-LCD fusion proteins were dialyzed overnight at room temperature against a dialyzing buffer containing 20 mM Tris–HCl pH 7.5, 200 mM NaCl, 20 mM BME, 0.5 mM EDTA, and 0.1 mM PMSF. The protein solutions were concentrated to 40–80 mg ml−1. The protein solutions (~100 µl) were filled into tightly sealed 1.5 ml silicon tubes, and the tubes were incubated at 4 °C for 1–3 days.
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