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2.9. RNA Sequencing (RNA-seq)
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The bovine preadipocytes were cultured in 10 cm cell culture plates. Cell samples were collected after induction of differentiation at 6 d. RNAs from three biological replicates of each group were isolated using RNeasy kit (Qiagen China (Shanghai) Co Ltd., Shanghai, China). The cell samples were subsequently sent to Novo Genefor transcriptome sequencing.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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