Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay

ZB Zhenzi Bai
XZ Xiaonan Zhao
CL Chenghua Li
CS Chuanlun Sheng
HL Hongyan Li
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The TUNEL fluorescence kit (Roche) was used for cell staining and assessment of cell death. 4′,6-Diamidino-2-phenylindole (DAPI, 1:5000; Beyotime, China) was used to stain the nuclei of cells. Cell death was assessed by calculating the number of TUNEL positive cells using a model SP8 laser scanning confocal microscope (Leica, Japan).

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