hiPSC reprograming.

Dermal fibroblasts were isolated from a 2-mm dermal punch biopsy from a 25-yr-old healthy male volunteer with no history of heart disease. Fibroblasts from the second passage were reprogrammed into hiPSCs using electroporation delivery (Neon) of nonintegrating episomal vectors (Epi5 Episomal iPSC Reprogramming Kit; ThermoFisher). After episomal delivery, fibroblasts were plated on a standard cell culture grade 10-cm plate. iPSC-like colonies were manually moved to hES-grade Matrigel (Corning)-coated 24-well plates between days 14 and 30 postelectroporation. Individual colonies were expanded for validation and line maintenance.