2.1. Dataset Preparation and Sequence Analysis

Publically available gene banks such as Ensembl (http://useast.ensembl.org/index.html), NCBI (http://www.ncbi.nlm.nih.gov/genbank), and UniProt (http://www.uniprot.org) were considered, but the NCBI database was used for coding the nucleotide and amino acid sequence of AKT3 for recovery and data analysis. The alignment of the sequences was performed with the help of Clustal Omega, in the MEGA 6.0 program [15]. Maximum likelihood methods were used to devise the phylogenetic tree within MEGA 6.0 for the AKT3 gene. Bootstrapping provided 1000 replicates for the clustering of taxa. The log likelihood of the topology and branch length indicated the number of substitutions per site [16, 17]. The species were identified by their accession number and their mRNA and protein accession numbers as listed in Table 1. The NCBI gene bank accession numbers for the mammalian gene AKT3 datasets were used for testing our hypothesis to construct various datasets.

List of species and accession number of the NCBI gene bank database, which was used for the hypothesis testing.