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Western blotting experiments were carried out as in our previous report [19]. The membrane was either incubated with anti-CCL2 antibody (1:500, ab25124, Abcam, Cambridge, UK) or anti-GAPDH antibody (1:200, sc-25778, Santa Cruz, CA, USA). All membranes were analyzed using ImageJ software (BIO-RAD, CA, USA). The CCL2 protein (~ 25 KD) expression was normalized to GAPDH (~ 37 KD).
Copyright and License information: Shanghai Institutes for Biological Sciences, CAS ©2019
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