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Porcine kidney-15 (PK-15) cells were maintained in minimum essential medium supplemented with 10% fetal bovine serum and incubated at 37 °C in 5% CO2. The three CSFV strains used in this study, comprised the two representative CSFV strains TD/96 and 94.4 and an attenuated lapinized vaccine strain LPC/AHRI, were propagated in the PK-15 cells [3,12,13].

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