2.3. RNA Isolation and Sequencing

Following the manufacture’s instruction, total RNA was extracted from the ovarian stroma of each individual using the RNeasy Mini Kit (Qiagen, Beijing, China). The RNA integrity was determined by an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). The RNAs with an average RIN value of 8.96 (7.8~10.0) were sent to generate libraries by Novogene (Novogene, Tianjin, China). All libraries were sequenced by the Novogene Illumina PE 150. RNA- and lncRNA-seq data used in the current study are available at the BioProject 598883 in NCBI (National Center for Biotechnology Information). The clean reads were obtained after the filtration of low-quality reads using standard quality control by FastaQC software.