CryoEM model building

To create a starting model of the 3D11 Fab-PbCSP complex, seven copies of 3D11 Fab/PbCSP-peptide crystal structures were manually docked into the 3D11 Fab-PbCSP cryoEM map using UCSF Chimera (Pettersen et al., 2004), followed by manual building using Coot (Emsley et al., 2010). All models were refined using phenix.real_space_refine (Adams et al., 2010) with secondary structure and geometry restraints. The final models were evaluated by MolProbity (Chen et al., 2010). The figures were prepared with UCSF Chimera (Pettersen et al., 2004) and UCSF ChimeraX (Goddard et al., 2018). Contacts in the 3D11 Fab-PbCSP complex were identified by PDBePISA (Krissinel and Henrick, 2007).