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Identification of positive spots by MALDI-TOF-MS
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Identification of the positive proteins were carried out according to our previous study (Li et al. 2015b). In brief, the detected protein spots were excised from the gels for digestion and subjected to MALDI-TOF–MS analysis. Peptide mass fingerprinting data were analyzed using the MASCOT server (http://www.matrixscience.com). Peptides with a rank of 1 in the MASCOT search were considered significant and used for the combined peptide score.
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