Host-associated yeast extractions.

C. elegans worms colonized with C. albicans were washed off the plate with 3 ml of M9 worm buffer. This suspension was centrifuged for 2 min at 2,000 rpm to pellet the worms. The supernatant was removed, and 1 ml of 3% bleach was added, transferred to a microcentrifuge tube, and incubated for 3 min. The worm suspension was centrifuged for 1 min at 12,000 rpm. The supernatant was removed, washed with 1 ml of M9, and centrifuged for 1 min at 12,000 rpm. The wash was repeated two more times to ensure that all bleach was removed. One-hundred-microliter aliquots of nematode suspension were transferred to 0.6-ml clear microtubes for manual disruption with a motorized pestle. After 1 min of manual disruption, the worm intestine solution was then diluted accordingly with an M9 buffer and plated on YPD plus 0.034 mg/liter chloramphenicol to select prevent any bacterial colonies from arising.