Protoplasts were isolated from 7-day-old etiolated “KeNong 199” seedlings. pGreen0800-LUC was fused with the TaLOX3 promoter and used as a reporter. 35S:TaWRKY42-B-GFP was used as an effector and 35S:GFP as a negative control. The plasmids pGreen0800-LUC and 35S:TaWRKY42-B-GFP or 35S:GFP, were co-transformed into wheat protoplasts and incubated for 18 h. The firefly and Renilla luciferase activity were quantified by the dual-luciferase assay kit (Promega, E1910).
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