The cellular activities of IL-6 and TNF-α were determined using ELISAs kits (IL-6, cat. no. 431304; TNF-α, cat. no. 430907) according to the manufacturers' instructions (BioLegend, Inc.). All samples were assayed six times.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.