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Analysis of reverse transcription products in infected cells
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MT-4 cells were grown in 24-well plates and infected with VSV-G pseudotyped pNL4-3 viruses (either WT or class II IN mutant) at a multiplicity of infection (MOI) of 2 in the presence of polybrene. After 6 hr, post-infection cells were collected, pelleted by brief centrifugation, and resuspended in PBS. DNA was extracted from cells using the DNeasy Blood and Tissue Kit (Qiagen) as per kit protocol. Quantity of HIV-1 vDNA was measured by Q-PCR using primers specific for early reverse-transcripts.
Copyright and License information: ©2020, Elliott et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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