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A total of 2000 cells/well were plated 12 h before treatment in 96-well plates. The cells were then handled for 72 h with specified agents. A total of 10% v/v of 5-mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution was introduced for a duration of 2 h. The medium was then discarded and the cells dissolved in DMSO (Sigma, St Louis, MO). Relative cytotoxicity was determined by using a BMG Labtek plate reader to measure the absorbance at 570 nm. All expected experiments were conducted in triplicates and mean was calculated with SEM.
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