Propidium iodide (PI) and fluorescein diacetate (FDA) staining

JH J. J. David Ho
NB Nathan C. Balukoff
PT Phaedra R. Theodoridis
MW Miling Wang
JK Jonathan R. Krieger
JS Jonathan H. Schatz
SL Stephen Lee
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Live cells were incubated with 2.5 μg/ml each of PI and FDA for 30 min at 37 °C and washed twice with media before imaging by fluorescence microscopy (Keyence, BZ-X800). Fixed cells were incubated with 10 μg/ml Hoechst 33342 (ThermoFisher Scientific) for 30 min at 37 °C and washed twice with PBS (−/−) before imaging by fluorescence microscopy. Cell were counted based on the number of Hoechst-stained nuclei per field.

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