Live cells were incubated with 2.5 μg/ml each of PI and FDA for 30 min at 37 °C and washed twice with media before imaging by fluorescence microscopy (Keyence, BZ-X800). Fixed cells were incubated with 10 μg/ml Hoechst 33342 (ThermoFisher Scientific) for 30 min at 37 °C and washed twice with PBS (−/−) before imaging by fluorescence microscopy. Cell were counted based on the number of Hoechst-stained nuclei per field.
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