Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
3.3. Analysis of Inhibitor Reversibility
Request a Protocol
Ask a
question
Favorite
The reversibilities of compounds COE-6, and COE-22 were analyzed while using a dialysis method after preincubating with MAO-B for 30 min, as previously described [54]. Reversibilities were determined for COE-6, COE-22, lazabemide (a reversible MAO-B reference inhibitor), and pargyline (an irreversible MAO-B reference inhibitor) at ~2 × IC50 concentrations, i.e., 0.004, 0.050, 0.080, and 0.040 µM, respectively. Relative activities of undialyzed (AU) and dialyzed (AD) samples were used to determine the reversibilities.
Copyright and License information: ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
