Fluorescence Imaging in vivo

RGD-Cy5.5-MBs were added into Eppendorf tubes and processed by ultrasound scanning using a high-frequency linear transducer (7 MHz, mechanical index 0.09), and then the fluorescence intensity was measured by a fluorescence imaging system. The groups with no ultrasound scanning and PBS were used as controls.

A total of 200 μL of RGD-Cy5.5-MBs were injected via the tail vein for fluorescence imaging in vivo, and nontargeted MBs were used as a control. The FX PRO in vivo fluorescence imaging system (Bruker, Switzerland) was used for scanning. Images of the mice were acquired prior to injection. Fluorescence scanning was then performed in 30-minute intervals to record the signal transitions of the tumours. The mice were sacrificed when the in vivo fluorescence signal was highly accumulated in the tumour area. The main organs and tumours were harvested for fluorescence scanning in vitro. Molecular imaging software (Bruker, Switzerland) was applied for quantitative analysis of the fluorescence intensity.