2.5. Measurement of TNF-α, IL-6, and Il-10 Protein Expression in Treated Macrophages

Following 24 h of antibiotic treatment, monocyte-derived macrophages were pelleted by centrifugation at 2500 rpm for 5 min at 4 °C. The supernatants were saved and TNF-α, IL-6, and Il-10 protein levels were determined using the following ELISA kits: Human TNF-α High Sensitivity ELISA, Human IL-6 High Sensitivity ELISA, and Human IL-10 High Sensitivity ELISA, all of which were purchased from Invitrogen (Carlsbad, CA, USA). All groups were tested in triplicate.