Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

4.4. Tartrate-Resistant Acid Phosphate (TRAP) Stain Assay

BL Binghan Li
YC Yuqing Chen
MZ Minghui Zhao
LZ Li Zuo
request Request a Protocol
ask Ask a question
Favorite

When the co-culturing or monoculturing was terminated, the cells were fixed and stained for TRAP using a phosphatase leukocyte kit (Sigma-Aldrich). The number of osteoclasts (TRAP-positive cells with more than three nuclei) and giant osteoclasts (osteoclasts with more than 20 nuclei) in every well was counted.

Copyright and License information:  ©2016 by the authors; licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A