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m6A-RIP qPCR

HZ Hui Zhang
XS Xinrui Shi
TH Tao Huang
XZ Xueni Zhao
WC Wanying Chen
NG Nannan Gu
RZ Rui Zhang
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RIP was performed as described above. Both the IP and input RNAs were reverse transcribed and the m6A marked mRNAs and NC (GAPDH) mRNA were detected by qPCR. The enrichment fold of IP versus input of each gene was calculated and normalized to NC. Primers were listed as follows:

ATP5C1-F GGGAGCTTCGGCGCAT

ATP5C1-R CGCGCGAGAGAACATGGTAG

DCTN1-F GCACGGTTCCTGACAAGTCTA

DCTN1-R GACACAGAATCCTGCTTGCC

PSMB4-F ATGGAAGCGTTTTTGGGGTC

PSMB4-R GAGTGGACGGAATGCGGTAA

SDHAF2-F GCCTTGCTTCCGGCTTCTTA

SDHAF2-R TGTCCATCACTTGAGGCAGG

GAPDH-F TGCCAAATATGATGACATCAAGAA

GAPDH-R GGAGTGGGTGTCGCTGTTG

Copyright and License information:  ©2020 The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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