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Mice were anesthetized by intraperitoneal injection with Ketamine/Xylazine (100 mg/kg and 10 mg/kg) and bleomycin (2.5 units/kg) (Sigma-Aldrich, St Louis, MO) in phosphate-buffered saline (PBS) or vehicle (PBS) alone was administered intratracheally (Sueblinvong et al., 2014a). At 7 or 14 days after bleomycin injection, the lungs were harvested and processed for Western blot analysis.
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