Cell Viability Assay

The drug mother liquor of 100 μg/mL was prepared in the medium containing 0.1% dimethyl sulfoxide (DMSO) as solvent. At the same time, the drug solution was diluted to 10, 20, 30, 40, and 50 μg/mL, respectively. Cells were inoculated into 96-well plates at a concentration of 2 × 10⁴ cells/well. Next, the cells were treated with various concentrations of drug solutions in triplicate. 24 and 48 h later, 20 μL of MTT (5 mg/mL) was added to each pore and the cells were cultured in darkness at 37°C for 4 h. After removing the equipartition samples, the remaining crystals (methyl sulfoxide precipitate) were dissolved with 150 μL of DMSO, the cells were cultured at 37°C for 10 min, and the absorbance (optical density [OD]) was measured with ELISA at 570 nm. Cell viability was determined by an MTT assay. The cell inhibition rate was calculated as follows: inhibition rate = [(OD_control − OD_treated)/OD_control] × 100%.