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Apoptotic Morphological Changes by Acridine Orange/Ethidium Bromide (AO/EtBr) Dual Staining
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Acridine orange/ethidium bromide (AO/EtBr) double staining was performed to assess apoptotic changes (Baskić et al., 2006). Briefly, control and CSME-treated MDA-MB-231 cells were stained with 4 μg/ml acridine orange and 4 μg/ml ethidium bromide for 15 min. The stained cells were examined under the fluorescence microscope in both red and green channels (Floid Cell Imaging System, Invitrogen).
Copyright and License information: ©2020 Maqbool, Sudharsan, Kanimozhi, Alrashood, Khan and Prasad.
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