Cervical specimens were collected for UU, MH, and CT testing. UU and MH were isolated by Mycoplasma IST 2 (BioMérieux), using the liquid culture method. Diagnostic criteria were based on the presence or absence of MH and UU and an estimate of concentration variation (cut-off 104-color-changing units-CCU/mL). CT was detected using an immunochromatography assay (Clearview; Unipath Ltd., Bedford, UK).
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