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Monodansylcadaverine (MDC) Staining

QQ Qing-Ming Qin
JP Jianwu Pei
GG Gabriel Gomez
AR Allison Rice-Ficht
TF Thomas A. Ficht
PF Paul de Figueiredo
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MDC staining was performed to evaluate the abundance of autophagic vacuoles in A. baumannii infected cells as previously described (Biederbick et al., 1995). Briefly, a 50 mM stock solution of MDC was prepared in dimethyl sulfoxide (DMSO). Cells were stained with MDC at a final concentration of 50 μM for 1 hr at room temperature, washed with phosphate-buffered saline for 3 × 10 min, and then examined by fluorescence microscopy.

Copyright and License information:  ©2020 Qin, Pei, Gomez, Rice-Ficht, Ficht and de Figueiredo.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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