4.10. Histopathological Examination

The testicles were obtained from the animals of the different groups and fixed in Bouin’s solution for 24 h. The tissue specimens were routinely processed to obtain 3–4 µm thick paraffin-embedded tissue sections. Tissue sections were stained with hematoxylin and eosin (H&E) stain following the method of Bancroft and Gamble (2008) [63]. A testicular scoring system was used according to the procedure reported earlier [64]. Thirty randomly selected seminiferous tubules were examined and evaluated in each experimental group. The score ranged from 10 to 1 according to presence of the main spermatogenic cells (spermatogonial cells, primary spermatocytes, secondary spermatocytes, and spermatid cells) and Sertoli cells.