4.7. Immunohistochemistry Localization and Quantification of OAT1, OAT3, and NFK-B Gene in the Kidney

An immunoperoxidase (peroxidase/antiperoxidase, PAP) protocol was employed to stain the kidney sections. OAT1 (ab131087, Abcam, Cambridge, UK), OAT3 (sc-293264, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and NFK-B (RB-9034-R7, Lab Vision, Fremont, CA, USA) antibodies were used at a dilution of 1:200. A light microscope was used to examine and photograph the slides, and quantification was performed with ImageJ software (ImageJ, 1.46a, NIH, USA).