Fibrinolysis (clot lysis) assay

Ten µL of each test compound or saline control was added to 240 µL of mouse plasma; 225 µL of this mixture was then added to 25 µL IIa and tPA in TBS/BSA containing 25 mM CaCl₂. The final concentration of IIa was 0.15 µg/ml, and of tPA was 1 µg/ml. Clot formation and lysis were monitored (OD₄₀₅) with a Molecular Devices microplate reader (SPECTRAmax 190) as described earlier [29,34].