2.6. CCK‐8 detection

Cells in the logarithmic phase were laid with the density of 1 × 10⁴ cells/well evenly, and the final volume of each well was 100 μL with the peripheral wells filled with PBS. The 96‐well plates were placed incubator, which was at 37°C and 5% CO₂. After cells attached to the wall, 10 μL of Cell Counting Kit‐8 (CCK‐8) reagent (Japan Tongren Chemical Research Institute, Dojindo, Japan) was added into each plate. After 2 hours of culture in the incubator, the absorbance value at 450 nm was measured in an enzyme, which labeled instrument at 24, 48, 72, and 96 hours, respectively, with the experimental results recorded.