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Peritoneal macrophages were isolated as previously described (Basso et al. 2009). A total of 3 μg/ml of LPS was added, and the cells were allowed to prime for 2 h. Subsequently, the cells were challenged with 1 mM of the P2X7R agonist BzATP for 30 min. Supernatants were collected and analyzed for interleukin 1 beta (IL-1β) using an enzyme-linked immunosorbent assay (ELISA) kit following the manufacturer’s instructions (Endogen, Pierce Technology, Rockford, IL, USA).
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Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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