2.4. Experiment 4: Adenosine A1 and A2A receptor agonist combination – sub-chronic administration

Thirty C58 mice were weaned and housed as described in Experiment 1. Repetitive behavior was quantified as described in Experiment 1. Each mouse was injected each day for seven days, although mice were only tested for repetitive behavior on days 1, 4, and 7. The A1 agonist (CPA at 0.3 mg/kg) and the A2A agonist ({"type":"entrez-protein","attrs":{"text":"CGS21680","term_id":"878113053","term_text":"CGS21680"}}CGS21680 at 0.3 mg/kg) were suspended in peanut oil and injected as described in Experiment 1.

Mice were randomly assigned to either the peanut oil vehicle group (n=15, 6 females, 9 males) or the adenosine A1 and A2A receptor agonist combination group (n=15, 7 females, 8 males). On days when repetitive behavior was not tested, each mouse was removed from the home cage, weighed, injected with either vehicle or the adenosine A1 and A2A receptor agonist combination (0.3 mg/kg of each drug) at 8:00PM and returned back to its home cage.

The total repetitive behavior counts in the six hours following injection were calculated for each mouse on each testing day (day 1, 4, and 7). Data were analyzed for main effects and interactions with a generalized linear model (SPSS Statistics 25) with sex, drug, and time as factors. We did not assess the effects of sub-chronic administration of the A1 and A2A receptor agonist combination on non-repetitive behaviors.