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WT and Slbp embryos were imaged from NC11 to NC13 mitosis with a Chk1 activity sensor (Deneke et al., 2016). Chk1 activity was determined from the cytoplasmic-to-nuclear intensity ratio of the Chk1 localization sensor in a 150×75 pixel area. The nuclear signal was taken from segmented nuclei eroded by 2 pixels to ensure nuclear signal was analyzed. The cytoplasmic signal was taken from inverted nuclear masks dilated by 2 pixels. Four embryos per genotype were analyzed.
Copyright and License information: ©2019 2019. Published by The Company of Biologists Ltd
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