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Cell Quantification

KK Karolina U. Kabayiza
GM Gauhar Masgutova
AH Audrey Harris
VR Vincent Rucchin
BJ Benvenuto Jacob
FC Frédéric Clotman
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Immunofluorescence images of cryosections were acquired on Zeiss Axio Cell Observer Z1 confocal microscope, EVOS FL or EVOS FL Auto Cell Imaging System. Brightness and contrast were adjusted uniformly in all replicate panels within an experiment with Adobe Photoshop CS3 software to match with observations. Cell quantification was performed using Adobe Photoshop CS3 software count analysis tool. Labeled neurons were counted on both sides of the spinal cord, on three sections for Onecut quantifications and on five sections for control/mutant comparisons, in brachial or thoracic regions from at least three pairs of control/mutant embryos. Raw data were exported from Adobe Photoshop CS3 software to SigmaPlot v11.0 software and processed to generate histogram figures and statistical analyses.

Copyright and License information:  ©2017 Kabayiza, Masgutova, Harris, Rucchin, Jacob and Clotman.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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