In vivo cancer growth of ovarian cancer in a xenograft model

An ovarian carcinoma xenograft model was employed to investigate the effect of KDM2B downregulation in tumorigenesis. Ten 5-week-old female nude mice were divided into two groups (five mice/group), which were subcutaneously injected with 1×10⁶ shKDM2B-A2780 cells or shNC-A2780 cells into the dorsal flank, respectively. Tumor volumes were measured every week, and after the mice had developed cachexia symptoms, xenografts were removed for further study. Each tumor was analyzed for KDM2B and EZH2 mRNA and protein levels by real-time quantitative PCR (RT-qPCR) and Western blot analysis. This animal experiment was approved by the Animal Experimental Ethics Committee of Guangxi Medical University. The care of laboratory animals and animal experimentation have conformed to the administration rules of laboratory animals of the Animal Experimental Ethics Committee of Guangxi Medical University.