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Determination of total antioxidant capacity (TAC)

BN Bakht Nasir
MB Muhammad Waleed Baig
MM Muhammad Majid
SA Syeda Masooma Ali
MK Muhammad Zafar Irshad Khan
SK Syeda Tayyaba Batool Kazmi
IH Ihsan-ul Haq
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Total antioxidant capacity of extracts was determined by phosphomolybdenum based method described previously [21]. The assay was performed by mixing 100 μl of extracts (4 mg/ml DMSO) with a reagent solution consisting of 28 mM sodium phosphate, 4 mM ammonium molybdate and 0.6 M sulphuric acid. Reaction mixture was then incubated at 95 °C for 90 min followed by cooling at room temperature. Absorbance was estimated at 695 nm using a microplate reader. The assay was executed in triplicate, ascorbic acid was used a positive control and calibration curve was drawn (y = 0.0096x + 0.1538, R2 = 0.9972). Negative control used in the experiment was DMSO and results were expressed as μg ascorbic acid equivalent (AAE) per mg extract.

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