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Quantitative real-time PCR

QN Quirino Alves de Lima Neto
FJ Francisco Ferreira Duarte Junior
PB Paulo Sérgio Alves Bueno
FS Flavio Augusto Vicente Seixas
MK Madzia Pauline Kowalski
EK Eyemen Kheir
TK Torsten Krude
MF Maria Aparecida Fernandez
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For analysis of chY RNAs expression in the GMA32 cell line, cDNA was synthesized from total RNA using random primers. The cDNA mixture was used as a template for the quantitative real-time PCR reaction (qPCR), which was performed in the iCycler iQ™ device, using the SYBR green supermix labeling kit (Bio-Rad) over 40 cycles and a hybridization temperature of 55 °C, as previously described [8]. For RNA-specific cDNA amplification, the primer sequences are provided in Additional file 6: Table S2.

Copyright and License information: Lima Neto et al. ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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