4.1. Western Blot

Cells were lysed in RIPA buffer (150 mM NaCl, 1.0% IGEPAL^® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris-HCl, pH 8.0). Lysates were separated via SDS-PAGE and transferred to a nitrocellulose (NC) membrane (GE Healthcare, Chicago, IL, USA). Blots were probed with the indicated primary antibodies, followed by Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG antibody, and detection was performed with a chemiluminescent HRP substrate (Thermo, Waltham, MA, USA). Antibodies for western blots are listed in Table S1.