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2.4. Vesicle Characterization by DLS and FETEM

YT Yen Wah Tong
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The size and zeta potential of the various polymeric vesicles were studied using a dynamic light scattering (DLS) Zetasizer Nano ZSP (Malvern Instrument Ltd., Malvern, UK) under different pH values in the range of pH 3 to 12. The stability of the vesicles was analyzed using a Malvern MPT-2 autotitrator, using a polymeric vesicle solution in a concentration of 0.05 mg/mL in 1 M PBS buffer. For the titration, 1 M PBS buffer solutions were prepared with pH values of 2, 7, and 12. The vesicular morphology was observed using a field emission transmission electron microscope (FETEM) JEM-2100F (JEOL, Tokyo, Japan). The samples were prepared by dropping 20 μL of 0.5 mg/mL vesicular solution on a copper grid and subsequent air drying.

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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