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2.8. Measurement of CYP2E1 activity
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The activity of CYP2E1 in freshly prepared liver microsome was estimated by colorimetrically measuring the formation of 4-nitrocatechol, a product from p-nitrophenol hydroxylation catalyzed specifically by CYP2E1. The protein concentration of CYP2E1 was measured by ELISA, and a rabbit anti-CYP2E1 antibody (Calbiochem, Inc., San Diego, CA, USA) was used for detection. The formed 4-nitrocatechol was expressed as nmol/mg protein.
Copyright and License information: China Medical University ©2016
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