32P‐labeling of RNAs

KN Kazumichi M Nishida
KS Kazuhiro Sakakibara
TS Tetsutaro Sumiyoshi
HY Hiroya Yamazaki
TM Taro Mannen
TK Takeshi Kawamura
TK Tatsuhiko Kodama
MS Mikiko C Siomi
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RNAs were purified from the beads using phenol/CHCl3. RNAs were then treated with Antarctic phosphatese (NEB) for dephosphorylation and radiolabeled using 32P‐gamma‐ATP by T4 polynucleotide kinase (NEB).

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