Haplotype (Hap) and nucleotide diversity (π) indices were computed in Arlequin v3.5.1.358 for each of the major rookeries in the Atlantic and Mediterranean Sea. Relationships among haplotypes were investigated in a neighbour-joining network using Network v4.6.1.3 (https://www.fluxus-engineering.com), and visualized with PopArt (https://popart.otago.ac.nz). Population structure amongst those major rookeries was investigated using FST estimates in Arlequin v3.5.1.3 (10.000 permutations). Because the role of Cabo Verde rookery is still yet to be clarified in the broader context of rookeries’ structure, we performed a hierarchic Analysis of Molecular Variance (AMOVA) considering different grouping scenarios, i.e. grouping Cabo Verde with all rookeries separately. The considered rookeries were Brazil, Mediterranean Sea, Mexico and USA. Most likely grouping was identified based on the FCT statistics.
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