2.8. Fatty Acid Methyl Ester (FAME) Analysis by Gas Chromatography/Mass Spectrometry

Cellular fatty acid methyl ester (FAME) analysis was performed by GC/MS, as previously described [59,60]. Fatty acid methyl esters were prepared as described by M. Sasser [61]. Briefly, fatty acid methyl esters were analyzed by gas chromatography/mass spectrometry (GC/MS). Compounds were separated using an Elite 5-MS column and monitored by mass spectrometry (Clarus 500—SQ 8 S, Perkin Elmer, Courtaboeuf, France). Spectral database search was performed using MS Search 2.0 operated with the Standard Reference Database 1A (NIST, Gaithersburg, MD, USA) and the FAMEs mass spectral database (Wiley, Chichester, UK).