Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

IgG Purification

PC Panjaporn Chaichana
BK Barbara Kronsteiner
PR Patpong Rongkard
PT Prapit Teparrukkul
DL Direk Limmathurotsakul
NC Narisara Chantratita
ND Nicholas P. J. Day
HF Helen A. Fletcher
SD Susanna J. Dunachie
request Request a Protocol
ask Ask a question
Favorite

Total IgG antibodies were individually purified from 100 μl of the plasma of each melioidosis patient in the cohort via negative selection using Melon™ Gel IgG Spin Purification Kit (Thermo Scientific, MA) according to the manufacturer's protocol. After purification, 82/136 patients in the cohort had sufficient concentration (≥ 3 mg/ml) of the antibodies for assays (n = 55 for survivors and n = 27 for non-survivors).

Copyright and License information:  ©2020 Chaichana, Kronsteiner, Rongkard, Teparrukkul, Limmathurotsakul, Chantratita, Day, Fletcher and Dunachie.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A