Treatments in mouse xenograft model

6-8 or 7-9 week-old female BALB/c nude and NOD/SCID mice were purchased from Shanghai Lingchang Biological Technology Co., Ltd, and were used for cell line derived xenograft (CDX) HCC models. Huh1 (5x10⁶ cells), HCCLM3 (5×10⁶ cells), Hep G2 (1×10⁷ cells), and PLCPRF5 (1×10⁷ cells) were inoculated subcutaneously on the right side of the mouse back, respectively. When tumor volume grew to approximately 150mm³, animals in each CDX study were randomly divided into 4 groups: control (10% DMSO) and gimatecan (0.1, 0.4, and 0.8mg/kg). Gimatecan and control were orally administered every four days, for a total of four times.