Caspase-3 activity assay

LC Ling Chen
SC Shang Cai
JW Jing-mei Wang
YH Ying-ying Huai
PL Pei-Hua Lu
QC Qian Chu
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Ovarian cancer cell lysates (20 μg of each treatment) were mixed with the described assay buffer16, together with the caspase-3 substrate (Ac-DEVD-AFC). Following extensive washes, the caspase-3 enzymatic AFC activity was measured at the excitation wavelength of 380 nm and the emission wavelength of 440 nm.

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