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Western blotting was performed as reported previously [17]. Bcl2-associated x protein (Bax), B-cell lymphoma-extra large (Bcl-xL), PI3K, phospho-PI3K, Akt, phospho-Akt, beclin-1, and autophagy gene 5 (ATG5), microtubule-associated protein light chain 3 II (LC3 II) and MDR1 protein expression were evaluated. The relative intensities were measured by a Biospectrum 810 AC Imaging System (UVP, Upland, CA, USA) and normalized to the β-actin control.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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